PRAKTIKUM ENZIM KATALASE pada HATI AYAM (BIOLOGI KLS XII)

Introduction

This tutorial provides a detailed guide on conducting an enzyme catalase experiment using chicken liver. The purpose of the experiment is to demonstrate how catalase works to neutralize hydrogen peroxide (H2O2), a toxic byproduct of metabolism, into harmless water (H2O) and oxygen (O2). Additionally, you'll learn about the factors affecting enzyme activity, such as temperature and pH.

Step 1: Gather Materials

Before starting the experiment, collect the following materials:

• Fresh chicken liver
• Hydrogen peroxide (H2O2) solution
• Test tubes
• Test tube rack
• Measuring cylinder
• Thermometer
• pH paper or pH meter
• Stopwatch or timer
• Knife and cutting board

Step 2: Prepare the Chicken Liver

• Cut the chicken liver into small pieces (about 1 cm cubes) to maximize surface area.
• Ensure that the liver pieces are fresh to obtain accurate results.

Step 3: Set Up the Experiment

• Place a few pieces of the chicken liver into a test tube.
• Measure a specific volume of hydrogen peroxide (e.g., 10 mL) using the measuring cylinder and add it to the test tube with the liver.
• Observe the reaction. You should see bubbles forming, indicating the release of oxygen gas.

Step 4: Test the Effects of Temperature

• Prepare multiple test tubes with chicken liver and hydrogen peroxide at different temperatures (e.g., room temperature, cold water, and warm water).
• Use the thermometer to monitor the temperature accurately.
• Record the rate of bubbling (oxygen production) at each temperature over a set period (e.g., 2 minutes).

Step 5: Test the Effects of pH

• Prepare test solutions with different pH levels (e.g., acidic, neutral, and alkaline) using pH paper or a pH meter.
• Add equal amounts of chicken liver and hydrogen peroxide to each test tube with the different pH solutions.
• Observe and record the rate of reaction in each case.

Step 6: Analyze Results

• Compare the rate of oxygen production at different temperatures and pH levels.
• Discuss the optimal conditions for catalase activity based on your observations.

Conclusion

In this experiment, you have successfully demonstrated how the enzyme catalase neutralizes hydrogen peroxide, observing the effects of temperature and pH on its activity. The key takeaways include:

• Enzyme activity can be influenced by environmental factors.
• Optimal conditions for enzyme function can enhance the breakdown of toxic substances.

For further exploration, consider experimenting with different substrates or enzyme sources to see how they compare with catalase from chicken liver.